Objective To assess the methylation level of MicroRNA-129-2 (MiR-129-2) in laryngeal cancer. Methods We applied Methyl-ProfilerTM DNA PCR Array System (SABiosciences Company) to analysis of 12 laryngeal cancer and 6 paracancer tissues. Results Hypermethylation was detected in 66.7% of the cancer tissue (8/12) and none in paracancer tissue (0/6) (P<0.05); the difference was significant. Among the clinical cancer tissue of each clinical stage , there was no significant difference of MiR-129-2 hypermethylation rate (P>0.05). There was no significant difference of MiR129-2 hypermethylation rate between two pathologic grades (P>0.05). Conclusion The methylation level of MiR1292 is higher in laryngeal cancer tissue than in paracancer tissue, indicating that it may play a role in laryngeal cancer. The methylation level of MiR-129-2 may not be related with the clinical stage of laryngeal cancer. The methylation level of MiR-129-2 may not be related with the pathologic grade of laryngeal cancer.