Objective: To analyze the audiological characteristics of patients with tympanosclerosis of different types, and to discuss the effect of surgical treatment, so as to provide reference for preoperative evaluation. Methods: 135 cases (135 ears) with tympanosclerosis were retrospectively analyzed, and the audiological data before and after operation were compared. Results: Conduction deafness was more common in type I and II tympanosclerosis patients (71.4% and 68.8%, respectively), and mixed deafness was more common in type IV tympanosclerosis patients (66.7%). The hearing curve of tympanosclerosis was mainly flat. The incidence of Kaha notch was 13.3% (18/135), and 38.9% of patients with type IV tympanosclerosis had Kaha notch on audiogram. In patients with all types of tympanosclerosis (except type III tympanosclerosis), the values of air bone conductance and the mean auditory threshold of air conductance were lower after surgery than before surgery. The success rate of surgery for type I tympanosclerosis was the highest (79%) when one of the criteria for surgical success was satisfied: Post-op ABG < 20dB, Post-op AC < 30dB, and ΔPost-op AC > 20dB. Tympanosclerosis IV had the lowest success rate (63.9%). Conclusion: The audiogram of patients with tympanosclerosis is mostly flat, and mixed deafness is most common in type IV tympanosclerosis, The appearance of the Kaha marks may indicate fixation of the stapes. Surgical removal of intractympanosclerosis is the main treatment method. The success rate is highest for type I tympanosclerosis and lowest for type IV tympanosclerosis.