Objective To evaluate the value of the combination of EB serological marker Rta protein IgG antibody (Rta / IgG), capsid antigen IgA antibody (VCA / IgA) ，early antigen IgA antibody (EA / IgA) in NPC screening in Linyi area. Methods From October 2019 to October 2022, the clinical data of 15873 subjects in physical examination group, 1800 subjects in clinical screening group with nasopharyngeal symptoms and 48 subjects in final diagnosis group with nasopharyngeal carcinoma confirmed by pathology in People's Hospital of Linyi Citywere collected,and draw the levels of the Rta / IgG, VCA / IgA and EA / IgA in the serum into the ROC curve and make statistical analysis to evaluate the diagnostic value of the three indexes and combined detection for nasopharyngeal carcinoma. Results The positive rates of Rta / IgG,VCA / IgA and EA / IgA in the confirmed group were 72.90% (35 / 48), 85.41% (41 / 48), 85.41% (41 / 48), respectively, In the clinical screening group, the positive rates were 17.17% (309 / 1800), 27.06% (487 / 1800), and 17.72% (319 / 1800), respectively, In the physical examination group, the positive rates were 10.34% (1642 / 15873),19.30% (3070 / 15873) , and 9.96% (1581 / 15873), respectively, The positive rate of the confirmed group was significantly higher than that of the clinical screening group（Χ2=95.918,78.029 and 136.589 , respectively, (Ｐ<0.01) and the physical examination group (Χ2=198.823, 132.894and 297.787, respectively, ( Ｐ<0.01), The differences were all statistically significant; Sensitivity of Rta / IgG,VCA / IgA and EA / IgA were 72.90%, 85.41%, 85.41%, respectively, The specificity were 89.0%, 79.9% and 89.2% , respectively. The sensitivity and specificity of the three antibodies were 95.83% (46 / 48) and 99.12% (17518 / 17673), respectively; and the area under the ROC curve AUC was 0.949, which was higher than the three antibodies alone (0.846,0.901 and 0.916). Conclusion The combined detection of Rta / IgG, VCA / IgA and EA / IgA can increase the antigen expression in the latency and lysis period of EB virus, which has a good complementary effect and significantly improves the detection rate of nasopharyngeal carcinoma.