Objective: To analyze the detection results of three antibodies related to EB virus serological markers, Rta protein IgG antibody (Rta/IgG), early antigen IgA antibody (EA/IgA), and capsid antigen IgA antibody (VCA/IgA), in the physical examination population, in order to provide theoretical references for early screening and prevention of nasopharyngeal carcinoma (NPC) in Linyi area. Method: Three antibody test results were collected from 15873 physical examination personnel in the physical examination center of Linyi People"s Hospital from October 2019 to October 2022.10,divide them into ≤ 30 year old group, 31-40 year old group, 41-50 year old group, 51-60 year old group, and ≥ 61 year old group based on age aggregation, and compare the three antibody absorbance values between each group, and statistical analysis was conducted using SPSS 26.0 software. Results The positive detection rates of Rta/IgG, EA/IgA, and VCA/IgA antibodies were 10.34%, 9.96%, and 19.30%, respectively, with a statistically significant difference (X2=780.030,Ｐ<0.05). When compared in different age groups (≤ 30 years old group, 31-40 years old group, 41-50 years old group, 51-60 years old group, ≥ 61 years old group), the overall antibody positive rate increased with age, The overall positive rates of VCA/IgA (16.76%, 18.81%, 18.85%, 19.06%, 31.61%) in different age groups were higher than those of Rta/IgG (8.54%, 8.91%, 9.89%, 11.05%, 11.63%) and EA/IgA (7.42%, 8.67%, 8.87%, 9.71%, 13.64%), with statistical significance (X2=18.957, 64.805, 16.222, respectively, (Ｐ<0.05); When comparing between genders, there were differences in Rta/IgG and VCA/IgA between genders (X2=5.479, 48.310, respectively, (Ｐ<0.05); When analyzing the positive aggregation of three antibodies, the proportions of 3 (-), 1 (+), 2 (+), and 3 (+) were 67.27%, 26.38%, 5.79%, and 0.56%, respectively, with statistically significant differences (X2=23319.137,Ｐ<0.05); Following 249 male and 137 female high-risk individuals, one male was diagnosed with NPC. Conclusion: The positive rate of EB virus serological testing in the physical examination population in Linyi City is related to age and gender. Risk assessment of the test results and tracking of high-risk populations are beneficial for early screening and prevention of NPC.