Objective: To investigate the expression of zinc finger protein ZNF114 in nasopharyngeal carcinoma tissues and the biological function of ZNF114 in nasopharyngeal carcinoma cells. Methods: The expression of ZNF114 in nasopharyngeal carcinoma in the Cancer Genome Atlas (TCGA) database was analyzed by bioinformatics methods, the expression of ZNF114 in nasopharyngeal carcinoma was verified by immunohistochemistry, and the effects of ZNF114 on the proliferation and migration invasion of nasopharyngeal carcinoma cells were confirmed by MTT, Scratch, EdU and Transwell assays. Results: ZNF114 was highly expressed in nasopharyngeal carcinoma, and was correlated with tumor size, lymph node metastasis, histological grade and clinical stage of nasopharyngeal carcinoma tissues. K-M survival analysis showed that low expression of ZNF114 had a better prognosis in OS, DSS and PFI of head and neck squamous cell carcinoma. KEGG function is mainly enriched in calcium signaling pathway, estrogen signaling pathway and primary immunodeficiency. Immunohistochemistry results showed that ZNF114 was highly expressed in nasopharyngeal carcinoma tissues and was associated with lymph node metastasis. Knockdown of ZNF114 can significantly inhibit the growth and migration and invasion of nasopharyngeal carcinoma cells. Conclusion: ZNF114 is involved in the proliferation and invasion of nasopharyngeal carcinoma and may be a predictive prognostic marker for nasopharyngeal carcinoma.