Objective To explore the influence of Eustachian tube function on hearing via analyzing the clinical data of patients with chronic suppurative otitis media undergoing endoscopic type I tympanoplasty.Methods A total of 42 patients with chronic suppurative otitis media were collected, and were divided into the study group (n=25) with Eustachian tube dysfunction and the control group (n=17) with normal Eustachian tube function based on the sound conduction anti-positive and negative pressure balance test and the Eustachian tube dysfunction scoring scale (ETDQ-7). The changes of hearing before and after tympanoplasty were compared between the two groups.Results The pre- and postoperative average hearing thresholds of air conduction and bone conduction in the study group were higher than those in the control group, and the differences were statistically significant (P<0.05). The improvement values of average thresholds of air conduction and bone conduction in the study group were higher than those in the control group, but the differences were statistically insignificant (P>0.05). The improvement value of air conduction threshold on 4 kHz of the study group was lower than that of the control group, but the difference was statistically insignificant (P>0.05). The improvement value of air conduction threshold on 8 kHz of the study group was lower than that of the control group, and the difference was statistically significant (P<0.05).Conclusion Good Eustachian tube function can not increase the degree of postoperative average hearing improvement in patients with chronic suppurative otitis media, but is beneficial to the recovery of high-frequency hearing.